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nr12s  (Tocris)
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A) Representative histograms from FACS analysis of indicated cell lines stained with Annexin V (left) or Duramycin (right). B) Quantification of percent positive cells for Annexin V (Left) or Duramycin (right). Data is from four independent experiments. C) Representative histogram of intracellular flow cytometry for TMEM30a-HA of indicated cell lines. D) Indicated cell lines were challenged with MNV CR6 at an MOI of 0.05 and viral titers enumerated 12 hours post-infection via plaque assay. Data is from three independent experiments. E) Indicated cell lines were either incubated either Annexin V or buffer alone on ice prior to performing a binding assay with MNV CR6 . Within an experiment binding was normalized to BV2 mock treated. Data is from three independent experiments. F) Ratio of the emission at 530 nm over 630 nm of indicated cell lines after incubated with <t>NR12S.</t> Data is from three independent experiments. All data are shown as mean ± S.D. from at least three independent experiments and analyzed by one-way ANOVA with Tukey’s multiple comparison test. Statistical significance is annotated as follows: ns not significant, * P < 0.05, ** P< 0.01, *** P< 0.001, **** P< 0.0001
Nr12s, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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diethylamino 5 oxo 5h benzo a phenoxazin 2 yl oxy propyl dodecyl  (Tocris)
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A) Representative histograms from FACS analysis of indicated cell lines stained with Annexin V (left) or Duramycin (right). B) Quantification of percent positive cells for Annexin V (Left) or Duramycin (right). Data is from four independent experiments. C) Representative histogram of intracellular flow cytometry for TMEM30a-HA of indicated cell lines. D) Indicated cell lines were challenged with MNV CR6 at an MOI of 0.05 and viral titers enumerated 12 hours post-infection via plaque assay. Data is from three independent experiments. E) Indicated cell lines were either incubated either Annexin V or buffer alone on ice prior to performing a binding assay with MNV CR6 . Within an experiment binding was normalized to BV2 mock treated. Data is from three independent experiments. F) Ratio of the emission at 530 nm over 630 nm of indicated cell lines after incubated with <t>NR12S.</t> Data is from three independent experiments. All data are shown as mean ± S.D. from at least three independent experiments and analyzed by one-way ANOVA with Tukey’s multiple comparison test. Statistical significance is annotated as follows: ns not significant, * P < 0.05, ** P< 0.01, *** P< 0.001, **** P< 0.0001
Diethylamino 5 Oxo 5h Benzo A Phenoxazin 2 Yl Oxy Propyl Dodecyl, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A) Representative histograms from FACS analysis of indicated cell lines stained with Annexin V (left) or Duramycin (right). B) Quantification of percent positive cells for Annexin V (Left) or Duramycin (right). Data is from four independent experiments. C) Representative histogram of intracellular flow cytometry for TMEM30a-HA of indicated cell lines. D) Indicated cell lines were challenged with MNV CR6 at an MOI of 0.05 and viral titers enumerated 12 hours post-infection via plaque assay. Data is from three independent experiments. E) Indicated cell lines were either incubated either Annexin V or buffer alone on ice prior to performing a binding assay with MNV CR6 . Within an experiment binding was normalized to BV2 mock treated. Data is from three independent experiments. F) Ratio of the emission at 530 nm over 630 nm of indicated cell lines after incubated with <t>NR12S.</t> Data is from three independent experiments. All data are shown as mean ± S.D. from at least three independent experiments and analyzed by one-way ANOVA with Tukey’s multiple comparison test. Statistical significance is annotated as follows: ns not significant, * P < 0.05, ** P< 0.01, *** P< 0.001, **** P< 0.0001
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benzo  (Tocris)
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A) Representative histograms from FACS analysis of indicated cell lines stained with Annexin V (left) or Duramycin (right). B) Quantification of percent positive cells for Annexin V (Left) or Duramycin (right). Data is from four independent experiments. C) Representative histogram of intracellular flow cytometry for TMEM30a-HA of indicated cell lines. D) Indicated cell lines were challenged with MNV CR6 at an MOI of 0.05 and viral titers enumerated 12 hours post-infection via plaque assay. Data is from three independent experiments. E) Indicated cell lines were either incubated either Annexin V or buffer alone on ice prior to performing a binding assay with MNV CR6 . Within an experiment binding was normalized to BV2 mock treated. Data is from three independent experiments. F) Ratio of the emission at 530 nm over 630 nm of indicated cell lines after incubated with <t>NR12S.</t> Data is from three independent experiments. All data are shown as mean ± S.D. from at least three independent experiments and analyzed by one-way ANOVA with Tukey’s multiple comparison test. Statistical significance is annotated as follows: ns not significant, * P < 0.05, ** P< 0.01, *** P< 0.001, **** P< 0.0001
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A) Representative histograms from FACS analysis of indicated cell lines stained with Annexin V (left) or Duramycin (right). B) Quantification of percent positive cells for Annexin V (Left) or Duramycin (right). Data is from four independent experiments. C) Representative histogram of intracellular flow cytometry for TMEM30a-HA of indicated cell lines. D) Indicated cell lines were challenged with MNV CR6 at an MOI of 0.05 and viral titers enumerated 12 hours post-infection via plaque assay. Data is from three independent experiments. E) Indicated cell lines were either incubated either Annexin V or buffer alone on ice prior to performing a binding assay with MNV CR6 . Within an experiment binding was normalized to BV2 mock treated. Data is from three independent experiments. F) Ratio of the emission at 530 nm over 630 nm of indicated cell lines after incubated with NR12S. Data is from three independent experiments. All data are shown as mean ± S.D. from at least three independent experiments and analyzed by one-way ANOVA with Tukey’s multiple comparison test. Statistical significance is annotated as follows: ns not significant, * P < 0.05, ** P< 0.01, *** P< 0.001, **** P< 0.0001

Journal: bioRxiv

Article Title: Membrane asymmetry facilitates murine norovirus entry and persistent enteric infection

doi: 10.1101/2024.11.06.622376

Figure Lengend Snippet: A) Representative histograms from FACS analysis of indicated cell lines stained with Annexin V (left) or Duramycin (right). B) Quantification of percent positive cells for Annexin V (Left) or Duramycin (right). Data is from four independent experiments. C) Representative histogram of intracellular flow cytometry for TMEM30a-HA of indicated cell lines. D) Indicated cell lines were challenged with MNV CR6 at an MOI of 0.05 and viral titers enumerated 12 hours post-infection via plaque assay. Data is from three independent experiments. E) Indicated cell lines were either incubated either Annexin V or buffer alone on ice prior to performing a binding assay with MNV CR6 . Within an experiment binding was normalized to BV2 mock treated. Data is from three independent experiments. F) Ratio of the emission at 530 nm over 630 nm of indicated cell lines after incubated with NR12S. Data is from three independent experiments. All data are shown as mean ± S.D. from at least three independent experiments and analyzed by one-way ANOVA with Tukey’s multiple comparison test. Statistical significance is annotated as follows: ns not significant, * P < 0.05, ** P< 0.01, *** P< 0.001, **** P< 0.0001

Article Snippet: 5 × 10 4 BV2 cells were seeded in black walled 96-well plates and the following day media was removed and 100 nM NR12S (Tocris) diluted in DMEM without FBS was added to cells at room temperature for 20 minutes.

Techniques: Staining, Flow Cytometry, Infection, Plaque Assay, Incubation, Binding Assay, Comparison